Last data update: Apr 29, 2024. (Total: 46658 publications since 2009)
Records 1-22 (of 22 Records) |
Query Trace: Lasker B[original query] |
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Updated review on Nocardia species: 2006-2021
Traxler RM , Bell ME , Lasker B , Headd B , Shieh WJ , McQuiston JR . Clin Microbiol Rev 2022 35 (4) e0002721 This review serves as an update to the previous Nocardia review by Brown-Elliott et al. published in 2006 (B. A. Brown-Elliott, J. M. Brown, P. S. Conville, and R. J. Wallace. Jr., Clin Microbiol Rev 19:259-282, 2006, https://doi.org/10.1128/CMR.19.2.259-282.2006). Included is a discussion on the taxonomic expansion of the genus, current identification methods, and the impact of new technology (including matrix-assisted laser desorption ionization-time of flight [MALDI-TOF] and whole genome sequencing) on diagnosis and treatment. Clinical manifestations, the epidemiology, and geographic distribution are briefly discussed. An additional section on actinomycotic mycetoma is added to address this often-neglected disease. |
Complete Genome Sequence of Rhodococcus sp. Strain W8901, a Human Clinical Specimen, Assembled Using MiSeq and MinION Sequence Data.
Gulvik CA , Batra D , Howard DT , Sheth M , Humrighouse BW , Lee J , McQuiston JR , Lasker BA . Microbiol Resour Announc 2021 10 (35) e0061321 Rhodococcus sp. strain W8901 is a Gram-positive, aerobic, mycolic acid-containing coccobacillus obtained from a patient with acute lymphocytic leukemia. Here, we report on the complete, circular genome sequence obtained using Illumina MiSeq and Oxford Nanopore Technologies MinION reads in order to better resolve the phylogeny of a rare pathogen. |
Complete and Circularized Bacterial Genome Sequence of Gordonia sp. Strain X0973
Gulvik CA , Batra D , Rowe LA , Sheth M , Nobles S , Lee JS , McQuiston JR , Lasker BA . Microbiol Resour Announc 2021 10 (9) Gordonia sp. strain X0973 is a Gram-positive, weakly acid-fast, aerobic actinomycete obtained from a human abscess with Gordonia araii NBRC 100433(T) as its closest phylogenetic neighbor. Here, we report using Illumina MiSeq and PacBio reads to assemble the complete and circular genome sequence of 3.75 Mbp with 3,601 predicted coding sequences. |
Complete and Circularized Genome Assemblies of the Kroppenstedtia eburnea Genus Type Strain and the Kroppenstedtia pulmonis Species Type Strain with MiSeq and MinION Sequence Data.
Gulvik CA , Batra D , Rowe LA , Sheth M , Humrighouse BW , Howard DT , Lee J , McQuiston JR , Lasker BA . Microbiol Resour Announc 2020 9 (44) Kroppenstedtia eburnea DSM 45196(T) and Kroppenstedtia pulmonis W9323(T) are aerobic, Gram-positive, filamentous, chemoorganotrophic thermoactinomycetes. Here, we report on the complete and circular genome assemblies generated using Illumina MiSeq and Oxford Nanopore Technologies MinION reads. Putative gene clusters predicted to be involved in the production of secondary metabolites were also identified. |
Draft Genome Sequence of Kroppenstedtia sanguinis X0209 T , a Clinical Isolate Recovered from Human Blood.
Arthur RA , Nicholson AC , Humrighouse BW , McQuiston JR , Lasker BA . Microbiol Resour Announc 2019 8 (24) Kroppenstedtia sanguinis X0209(T), a thermoactinomycete, was isolated from the blood of a patient in Sweden. We report on the draft genome sequence obtained with an Illumina MiSeq instrument. The assembled genome totaled 3.73 Mb and encoded 3,583 proteins. Putative genes for virulence, transposons, and biosynthetic gene clusters have been identified. |
Streptacidiphilus bronchialis sp. nov., a ciprofloxacin-resistant bacterium from a human clinical specimen; reclassification of Streptomyces griseoplanus as Streptacidiphilus griseoplanus comb. nov. and emended description of the genus Streptacidiphilus.
Nouioui I , Klenk HP , Igual JM , Gulvik CA , Lasker BA , McQuiston JR . Int J Syst Evol Microbiol 2019 69 (4) 1047-1056 The taxonomic position of strain 15-057A(T), an acidophilic actinobacterium isolated from the bronchial lavage of an 80-year-old male, was determined using a polyphasic approach incorporating morphological, phenotypic, chemotaxonomic and genomic analyses. Pairwise 16S rRNA gene sequence similarities calculated using the GGDC web server between strain 15-057A(T) and its closest phylogenetic neighbours, Streptomyces griseoplanus NBRC 12779(T) and Streptacidiphilus oryzae TH49(T), were 99.7 and 97.6 %, respectively. The G+C content of isolate 15-057A(T) was determined to be 72.6 mol%. DNA-DNA relatedness and average nucleotide identity between isolate 15-057A(T) and Streptomyces griseoplanus DSM 40009(T) were 29.2+/-2.5 % and 85.97 %, respectively. Chemotaxonomic features of isolate 15-057A(T) were consistent with its assignment within the genus Streptacidiphilus: the whole-cell hydrolysate contained ll-diaminopimelic acid as the diagnostic diamino acid and glucose, mannose and ribose as cell-wall sugars; the major menaquinone was MK9(H8); the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, glycophospholipid, aminoglycophospholipid and an unknown lipid; the major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. Phenotypic and morphological traits distinguished isolate 15-057A(T) from its closest phylogenetic neighbours. The results of our taxonomic analyses showed that strain 15-057A(T) represents a novel species within the evolutionary radiation of the genus Streptacidiphilus, for which the name Streptacidiphilus bronchialis sp. nov. is proposed. The type strain is 15-057A(T) (=DSM 106435(T)=ATCC BAA-2934(T)). |
Complete Genome Sequence of Nocardia farcinica W6977 T Obtained by Combining Illumina and PacBio Reads.
Gulvik CA , Arthur RA , Humrighouse BW , Batra D , Rowe LA , Lasker BA , McQuiston JR . Microbiol Resour Announc 2019 8 (3) The complete genome sequence of the Nocardia farcinica type strain was obtained by combining Illumina HiSeq and PacBio reads, producing a single 6.29-Mb chromosome and 2 circular plasmids. Bioinformatic analysis identified 5,991 coding sequences, including putative genes for virulence, microbial resistance, transposons, and biosynthesis gene clusters. |
Complete Genome Sequence of Streptacidiphilus sp. Strain 15-057A, Obtained from Bronchial Lavage Fluid.
Arthur RA , Gulvik CA , Humrighouse BW , Lasker BA , Batra D , Rowe LA , Igual JM , Nouioui I , Klenk HP , McQuiston JR . Microbiol Resour Announc 2018 7 (19) Streptacidiphilus sp. strain 15-057A was isolated from a bronchial lavage sample and represents the only member of the genus not isolated from acidic soils. A single circular chromosome of 7.01 Mb was obtained by combining Illumina and PacBio sequencing data. Bioinformatic analysis detected 63 putative secondary biosynthetic gene clusters and recognized 43 transposons. |
Kroppenstedtia pulmonis sp. nov. and Kroppenstedtia sanguinis sp. nov., isolated from human patients.
Bell ME , Lasker BA , Klenk HP , Hoyles L , Sproer C , Schumann P , Brown JM . Antonie Van Leeuwenhoek 2016 109 (5) 603-10 Three human clinical strains (W9323T, X0209T and X0394) isolated from a lung biopsy, blood and cerebral spinal fluid, respectively, were characterised using a polyphasic taxonomic approach. Comparative analysis of the 16S rRNA gene sequences showed the three strains belong to two novel branches within the genus Kroppenstedtia: 16S rRNA gene sequence analysis of W9323T showed close sequence similarity to Kroppenstedtia eburnea JFMB-ATET (95.3 %), Kroppenstedtia guangzhouensis GD02T (94.7 %) and strain X0209T (94.6 %); sequence analysis of strain X0209T showed close sequence similarity to K. eburnea JFMB-ATET (96.4 %) and K. guangzhouensis GD02T (96.0 %). Strains X0209T and X0394 were 99.9 % similar to each other by 16S rRNA gene sequence analysis. The DNA-DNA relatedness was 94.6 %, confirming that X0209T and X0394 belong to the same species. Chemotaxonomic data for strains W9323T and X0209T were consistent with those described for the members of the genus Kroppenstedtia: the peptidoglycan was found to contain LL-diaminopimelic acid; the major cellular fatty acids were identified as iso-C15 and anteiso-C15; and the major menaquinone was identified as MK-7. Differences in endospore morphology, carbon source utilisation profiles, and cell wall sugar patterns of strains W9323T and X0209T, supported by phylogenetic analysis, enabled us to conclude that the strains each represent a new species within the genus Kroppenstedtia, for which the names Kroppenstedtia pulmonis sp. nov. (type strain W9323T = DSM 45752T = CCUG 68107T) and Kroppenstedtia sanguinis sp. nov. (type strain X0209T = DSM 45749T = CCUG 38657T) are proposed. |
Nocardia arizonensis sp. nov., obtained from human respiratory specimens.
Lasker BA , Bell M , Klenk HP , Schumann P , Brown JM . Antonie Van Leeuwenhoek 2015 108 (5) 1129-37 In 2008, three clinical isolates (W9405T, W9409 and W9575) were obtained from bronchial wash or sputum specimens from patients from the state of Arizona and characterised by polyphasic analysis. All three clinical isolates 16S rRNA gene sequences were found to be 100 % identical to each other and showed the strains belong in the genus Nocardia. BLASTn searches in the GenBank database of near full-length 16S rRNA gene sequences showed the highest sequence similarities to the type strains of Nocardia takedensis (98.3 %, sequence similarity), Nocardia lijiangensis (97.4 %), Nocardia harenae (97.4 %), and Nocardia xishanensis (97.1 %). The DNA-DNA relatedness between isolate W9405T and the type strain of N. takedensis is 26.0 +/- 2.4 % when measured in silico using genomic DNA sequences. The G+C content of isolate W9405T is 68.6 mol%. Chemotaxonomic analyses of the clinical isolates were consistent with their assignment to the genus Nocardia: whole cell hydrolysates contain meso-diaminopimelic acid as the diagnostic diamino acid of peptidoglycan; the whole-cell sugars are arabinose and galactose; the predominant phospholipids include diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol; MK-8-(H4) omega-cyc as the major menaquinone; mycolic acids ranging from 38 to 62 carbon atoms; and palmitic acid, tuberculostearic acid, palmitelaidic acid and oleic acid are the major fatty acids. Genus and species specific profiles were obtained following analysis by matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra of the clinical isolates. All isolates were found to be intermediately resistant or resistant to minocycline and resistant to ciprofloxacin but were susceptible to amikacin, imipenem and linezolid. Our polyphasic analysis suggest the three clinical isolates obtained from patients in Arizona represent a novel species of Nocardia for which we propose the name Nocardia arizonensis, with strain W9405T (=DSM 45748T = CCUG 62754T = NBRC 108935T) as the type strain. |
Nocardia vulneris sp. nov., isolated from wounds of human patients in North America.
Lasker BA , Bell M , Klenk HP , Sproer C , Schumann P , Brown JM . Antonie Van Leeuwenhoek 2014 106 (3) 543-53 Nocardia species are ubiquitous in the environment with an increasing number of species isolated from clinical sources. From 2005 to 2009, eight isolates (W9042, W9247, W9290, W9319, W9846, W9851T, W9865, and W9908) were obtained from eight patients from three states in the United States and Canada; all were from males ranging in age from 47 to 81 years old; and all were obtained from finger (n = 5) or leg (n = 3) wounds. Isolates were characterized by polyphasic analysis using molecular, phenotypic, morphologic and chemotaxonomic methods. Sequence analysis of 16S rRNA gene sequences showed the eight isolates are 100 % identical to each other and belong in the genus Nocardia. The nearest phylogenetically related neighbours were found to be the type strains for Nocardia altamirensis (99.33 % sequence similarity), Nocardia brasiliensis (99.37 %), Nocardia iowensis (98.95 %) and Nocardia tenerifensis (98.44 %). The G+C content of isolate W9851T was determined to be 68.4 mol %. The DNA-DNA relatedness between strain W9851T and the N. brasiliensis type strain was 72.8 % and 65.8 % when measured in the laboratory and in silico from genome sequences, respectively, and 95.6 % ANI. Whole-cell peptidoglycan was found to contain meso-diaminopimelic acid; MK-8-(H4)omega-cyc was identified as the major menaquinone; the major fatty acids were identified as C16:0, 10 Me C18:0, and C18:1 w9c, the predominant phospholipids were found to include diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; whole-cell sugars detected were arabinose and galactose; and mycolic acids ranging from 38 to 60 carbon atoms were found to be present. These chemotaxonomic analyses are consistent with assignment of the isolates to the genus Nocardia. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra of the clinical isolates showed genus and species level profiles that were different from other Nocardia species. All isolates were resistant to ciprofloxacin, clarithromycin and imipenem but were susceptible to amikacin, amoxicillin/clavulanate, linezolid and trimethoprim/sulfamethoxazole. The results of our polyphasic analysis suggest the new isolates obtained from wound infections represent a novel species within the genus Nocardia, for which the name Nocardia vulneris sp. nov. is proposed, with strain W9851T (= DSM 45737T = CCUG 62683T = NBRC 108936T) as the type strain. |
Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: broad genetic diversity and a cluster of related strains in one NICU.
Reiss E , Lasker BA , Lott TJ , Bendel CM , Kaufman DA , Hazen KC , Wade KC , McGowan KL , Lockhart SR . Infect Genet Evol 2012 12 (8) 1654-60 Candida parapsilosis (CP) (n=40) isolated from an unselected patient population in the neonatal intensive care units (NICUs) of 3 U.S. hospitals were collected over periods of 3.5-9 years. Two previously published microsatellite markers and three additional trinucleotide markers were used to produce multiplex genotypes, which revealed broad strain diversity among the NICU isolates with a combined index of discrimination (D)=0.997. A cluster of 8 related CP strains from 4 infants in a single NICU was observed. An extended collection of 24 CP isolates from the general population of that hospital showed that the cluster of NICU isolates was related to 3 isolates from general hospital patients. This microsatellite marker set is suitable to investigate clusters of colonizing and infecting strains of CP. |
Pattern of antimicrobial susceptibility obtained from blood isolates of a rare but emerging human pathogen, Gordonia polyisoprenivorans
Moser BD , Pellegrini GJ , Lasker BA , Brown JM . Antimicrob Agents Chemother 2012 56 (9) 4991-3 The genus Gordonia, originally described in 1971 by Tsukamura, consisted of both clinical and environmental isolates (9).... |
Nocardia amikacinitolerans sp. nov., an amikacin-resistant human pathogen.
Ezeoke I , Klenk HP , Potter G , Schumann P , Moser BD , Lasker BA , Nicholson A , Brown JM . Int J Syst Evol Microbiol 2012 63 1056-1061 Five isolates from clinical human sources were evaluated. Analysis of the near full length 16S rRNA gene showed 99.9-100 % similarity among the strains. The results of a comparative phylogenetic analysis of the 16S rRNA gene sequences indicated that the isolates belonged to the genus Nocardia. Phenotypic and molecular analyses were performed on the clinical isolates. Traditional phenotypic analyses included morphologic, biochemical/physiological, chemotaxonomic and antimicrobial susceptibility profiling. Molecular studies included 1441-bp 16S rRNA and 1246-bp gyrB gene sequence analyses, as well as DNA-DNA hybridizations. Biochemical analysis failed to differentiate the putative novel species from its phylogenetic neighbors; however, molecular studies were able to distinguish the patient strains and confirm them as a single species. Based on 16S rRNA gene sequence analysis, similarity between the isolates and their closest relatives (Nocardia araoensis, Nocardia arthritidis, Nocardia beijingensis and Nocardia niwae) were less than or equal to 99.3 %. Partial gyrB gene sequence analysis showed 98-99.7 % relatedness among the isolates. Nocardia lijiangensis and Nocardia xishanensis were the isolates' closest related species based on gyrB gene sequence analysis and showed 95.7 and 95.3 % similarity, respectively. Resistance to amikacin and molecular analyses, including DNA-DNA hybridization, distinguished the five patient strains from their phylogenetic neighbors, and the results of this polyphasic study indicated a novel species of Nocardia for which we propose the name Nocardia amikacinitolerans sp. nov., with strain W9988T (=DSM 45539 T = CCUG 59655T) as the type strain. |
Nocardia cyriacigeorgica infections attributable to unlicensed cosmetic procedures -- an emerging public health problem?
Apostolou A , Bolcen SJ , Dave V , Jani N , Lasker BA , Tan CG , Montana B , Brown JM , Genese CA . Clin Infect Dis 2012 55 (2) 251-3 We describe an outbreak of Nocardia cyriacigeorgica soft-tissue infections attributable to unlicensed cosmetic injections and the first report using MLST sequence data for determining Nocardia strain relatedness in an outbreak. All eight cases identified had a common source exposure and required hospitalization, surgical debridement, and prolonged antimicrobial therapy. |
The threat of artemisinin-resistant malaria
Dondorp AM , Fairhurst RM , Slutsker L , Macarthur JR , M DJg , Guerin PJ , Wellems TE , Ringwald P , Newman RD , Plowe CV . N Engl J Med 2011 365 (12) 1073-5 In the 1970s, Chinese government scientists working on a secret “Project 523” developed a new class of potent antimalarial drugs, the artemisinins or qinghaosu derivatives. In mostly unpublished work that has just been recognized by a 2011 Lasker Award to Tu Youyou, researchers in China isolated the active compounds from the plant Artemisia annua, tested them in mice, analyzed the chemical structure of the artemisinins, and demonstrated their high potency and rapid efficacy in human trials. Although they were widely used in China during the 1980s, only in the 1990s did the artemisinins come to wider global attention in the form of artemisinin-based combination therapies. Over the past decade, these highly efficacious treatments, along with other malaria-control measures, have contributed to significant reductions of the malaria burden in many areas of the world, including parts of Africa. | Together, these successes and increased funding have revived the bold aspiration to eradicate malaria. About one quarter of malaria-afflicted countries are already shifting their focus from malaria control to elimination. Past successful malaria-elimination schemes have all depended on reliable curative drugs, used in conjunction with vector-control methods. Similarly, current elimination plans rely on the long-term availability of effective antimalarial drugs — a requirement that is pivotally dependent on the efficacy of artemisinins. The artemisinin derivative artesunate has also proven to be the best drug against severe falciparum malaria. Losing the artemisinins to resistance would be a disaster for the control and treatment of malaria and would bring eradication efforts to a standstill. |
Characterization of human clinical isolates of Dietzia species previously misidentified as Rhodococcus equi.
Niwa H , Lasker BA , Hinrikson HP , Franzen CG , Steigerwalt AG , Whitney AM , Brown JM . Eur J Clin Microbiol Infect Dis 2011 31 (5) 811-20 In this study, 16 human clinical isolates of Dietzia species previously misidentified as Rhodococcus equi were evaluated using phenotypic methods, including traditional and commercial (API Coryne) biochemical tests, antimicrobial susceptibility testing, and 16S rRNA gene and gyrB gene sequencing. Positive results for both the hydrolysis of adenine and Christie-Atkins-Munch-Petersen (CAMP) reaction allowed for differentiation between the Dietzia isolates and the type strain of Rhodococcus equi; however, traditional and commercial phenotypic profiles could not be used to reliably identify Dietzia species. The analysis of 16S rRNA gene and gyrB gene sequences could discriminate all Dietzia strains from the type strain of R. equi. Most Dietzia species had distinct 16S rRNA gene and gyrB gene sequences; however, the 16S rRNA gene sequences of the type strains of D. schimae and D. cercidiphylli were identical to D. maris and D. natronolimnaea, respectively. Based on comparative sequence analysis, five clinical isolates clustered with D. maris/D. schimae and nine with D. natronolimnaea/D. cercidiphylli. The two remaining isolates were found to be most closely related to the D. cinnamea/D. papillomatosis clade. Even though molecular analyses were not sufficiently discriminative to accurately identify all Dietzia species, the method was able to reliably identify isolates that were previously misidentified by phenotypic methods to the genus level. |
Gordonia bronchialis bacteremia and pleural infection: case report and review of the literature
Johnson JA , Onderdonk AB , Cosimi LA , Yawetz S , Lasker BA , Bolcen SJ , Brown JM , Marty FM . J Clin Microbiol 2011 49 (4) 1662-6 Gordonia species are aerobic actinomycetes recently recognized as causing human disease, often in the setting of intravascular catheter-related infections. We describe a case of Gordonia bronchialis bacteremia and pleural space infection in the absence of an indwelling intravascular catheter, and review the breadth of reported infections with this emerging pathogen. |
Mutant selection window and characterization of the allelic diversity for ciprofloxacin resistant mutants of Rhodococcus equi
Niwa H , Lasker BA . Antimicrob Agents Chemother 2010 54 (8) 3520-3 The mutant prevention concentration (MPC) for ciprofloxacin was determined for two Rhodococcus equi strains. The MPC for both strains was 32 mug/ml, which is above the peak serum concentration of ciprofloxacin obtainable by oral administration in humans. Nine single nucleotide changes corresponding to eight amino acid substitutions in the quinolone resistance-determining regions of DNA gyrase subunit A and B were characterized. Only mutants with amino acid changes in Ser-83 of GyrA were highly resistant (≥64 mug/ml). Our results suggest that ciprofloxacin monotherapy against R. equi infection may result in the emergence of ciprofloxacin resistant mutants. |
Investigation of an apparent outbreak of Rhodococcus equi bacteremia
Langer AJ , Feja K , Lasker BA , Hinrikson HP , Morey RE , Pellegrini GJ , Smith TL , Robertson C . Diagn Microbiol Infect Dis 2010 67 (1) 95-100 During January to April 2007, hospital staff reported 3 patients with Rhodococcus equi bloodstream infections. Isolates were analyzed at the Centers for Disease Control and Prevention, Atlanta, GA, to confirm identification and to assess strain relatedness; 2 were R. equi but genetically distinct, and 1 was identified as Gordonia polyisoprenivorans. Rapid reference laboratory support prevented an unnecessary outbreak investigation. |
Nocardia niwae sp. nov., isolated from human pulmonary sources.
Moser BD , Klenk HP , Schumann P , Potter G , Lasker BA , Steigerwalt AG , Hinrikson HP , Brown JM . Int J Syst Evol Microbiol 2010 61 438-442 Members of the genus Nocardia are responsible for cutaneous, pulmonary and disseminated human infections. From 2003 to 2008, four nocardioform strains (W8027, W8681, W9071, W9241T) were isolated from persons in the state of Florida, USA. Ribosomal gene sequencing analysis suggested that a novel Nocardia species had been isolated. These strains underwent polyphasic taxonomic analysis. Phenotypic analyses included morphologic examination, biochemical profiling and antimicrobial susceptibility testing. Molecular studies included 16S rRNA and DNA gyrase B subunit (gyrB) gene sequence analyses and DNA-DNA hybridization. Phylogenetic neighbours were determined through 16S rRNA and gyrB gene sequence analyses. Differential phenotypic characteristics of the novel Nocardia species compared to phylogenetically related species were growth at 45C and 3 out of 4 novel strains utilized L-rhamnose. The antimicrobial profiles could not reliably distinguish the novel species from related nocardiae. Analysis showed that the 16S rRNA gene sequences of the four novel isolates were identical. The BLAST analysis of the near full length 16S rRNA gene showed 99.2 % sequence similarity to N. araoensis DSM 44729T, N. arthritidis DSM 44731T and N. beijingensis JCM 10666 T, 98.7 % to N. amamiensis DSM 45066T, 98.2 % to N. pneumoniae JCM 12119T and 97.8 % to N. takedensis JCM 13313T; the analysis of partial gyrB gene sequences showed 95.4 % similarity to N. arthritidis DSM 44731T, 95.3 % to N. gamkensis DSM 44956T, 94.4 % to N. pneumoniae JCM 12119T, 93.8 % to asiatica DSM44668T, 93.5 % to N. amamiensis DSM 45066T, 93.4 % to N. beijingensis JCM 10666 T and 93.2 % to N. araoensis DSM 44729T. The DNA-DNA hybridization percentages among the four novel strains were 86-89 %; the hybridization percentages of W9241T compared to N. beijingensis JCM 10666T was 47 %, to N. araoensis DSM 44729T was 46 %, to N. arthritidis DSM 44731T was 44 %, to N. amamiensis DSM 45066T was 32 % and to N. asiatica DSM 44668T was 20 %. The results of our polyphasic taxonomic analysis suggested that a novel species of Nocardia was identified for which we propose the name Nocardia niwae sp. nov. The type strain is W9241T ( = DSM 45340T = CCUG 57756T). |
Nocardia mikamii sp. nov., isolated from human pulmonary infections in the United States
Jannat-Khah DP , Kroppenstedt RM , Klenk HP , Sproer C , Schumann P , Lasker BA , Steigerwalt AG , Hinrikson HP , Brown JM . Int J Syst Evol Microbiol 2009 60 (10) 2272-2276 Four nocardioform bacteria were isolated from clinical respiratory sources (W7467, W7811, W8061T and W9013) in the United States. Macroscopic examination showed scant aerial hyphae and beige-red substrate hyphae. They showed chemotaxonomic markers that were consistent with the classification of Nocardia: i.e., meso-diaminopimelic acid; arabinose and galactose as diagnostic sugars; the phospholipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides; a menaquinone with a omega-cyclic isoprene side chain MK-8(H4cycl.); a fatty acid pattern composed of unbranched saturated and monounsaturated fatty acids with considerable amount of tuberculostearic acid; and mycolic acids comprising 52 to 62 carbon atoms with three principal mycolic acids which were mono- and polyunsaturated showing a chain length of C54, C56 and C58. 16S rRNA gene sequence data and phenotypic characters showed they were most closely related to Nocardia aobensis (DSM 44805T ). The G+C content was 68.3 mol %. Analysis of a 1,245-bp fragment of gyrB gene showed a clade separate from N. aobensis and was supported by the DNA relatedness of 67 % of W8061T to N. aobensis. These data indicated that the novel isolates represent a new species within the genus Nocardia, for which the name Nocardia mikamii sp. nov. is proposed, with W8061T (DSM 45174T = JCM 15508T) designated as the type strain. |
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